Matches in SemOpenAlex for { <https://semopenalex.org/work/W2364001374> ?p ?o ?g. }
Showing items 1 to 55 of
55
with 100 items per page.
- W2364001374 abstract "To use paper cellulose as a carrier,immobilization of papain on paper cellulose was studied.Thus,papain was immobilized onto glutaraldehyde-activated paper cellulose under acid conditions.The enzymatic properties,such as proteolytic activity,heat-stability,glutaraldehyde volume fraction,enzyme amount and pH-activity profile,of the immobilized papain preparation were examined.The immobilization conditions were optimized by orthogonal experiments.The prepared products were applied to digest anti-HCG to obtain F(ab′)2 fragments.The results indicated that the immobilized enzyme has an high activity at 40 ℃ when the pH of the buffer solution is 5.0 as well as with the glutaraldehyde volume fraction is 0.01 %.Besides the enzyme concentration is 46 mg/g.The activity of the products can reach up to 19 250 U/g which are ten times higher than those came from the processes not optimized.The preparation and characterization of the F(ab′)2 fragments of anti-HCG are presented in detail.The monoclonal antibody was digested with immobilized papain to produce antibody fragment.The F(ab′)2 fragments were then purified by using DEAE-FF size exclusion chromatography.The F(ab′)2 fragment(110 KDa) maintained potent suppressive activity and the titer can reaches 1∶12 000." @default.
- W2364001374 created "2016-06-24" @default.
- W2364001374 creator A5054756337 @default.
- W2364001374 date "2013-01-01" @default.
- W2364001374 modified "2023-09-22" @default.
- W2364001374 title "Study on the paper cellulose immobilized papain and its exploration" @default.
- W2364001374 hasPublicationYear "2013" @default.
- W2364001374 type Work @default.
- W2364001374 sameAs 2364001374 @default.
- W2364001374 citedByCount "0" @default.
- W2364001374 crossrefType "journal-article" @default.
- W2364001374 hasAuthorship W2364001374A5054756337 @default.
- W2364001374 hasConcept C181199279 @default.
- W2364001374 hasConcept C185592680 @default.
- W2364001374 hasConcept C2777529624 @default.
- W2364001374 hasConcept C2779251873 @default.
- W2364001374 hasConcept C2779832904 @default.
- W2364001374 hasConcept C28212737 @default.
- W2364001374 hasConcept C33633552 @default.
- W2364001374 hasConcept C43617362 @default.
- W2364001374 hasConcept C55493867 @default.
- W2364001374 hasConceptScore W2364001374C181199279 @default.
- W2364001374 hasConceptScore W2364001374C185592680 @default.
- W2364001374 hasConceptScore W2364001374C2777529624 @default.
- W2364001374 hasConceptScore W2364001374C2779251873 @default.
- W2364001374 hasConceptScore W2364001374C2779832904 @default.
- W2364001374 hasConceptScore W2364001374C28212737 @default.
- W2364001374 hasConceptScore W2364001374C33633552 @default.
- W2364001374 hasConceptScore W2364001374C43617362 @default.
- W2364001374 hasConceptScore W2364001374C55493867 @default.
- W2364001374 hasOpenAccess W2364001374 @default.
- W2364001374 hasRelatedWork W1123897411 @default.
- W2364001374 hasRelatedWork W2022836504 @default.
- W2364001374 hasRelatedWork W2024353929 @default.
- W2364001374 hasRelatedWork W2075727320 @default.
- W2364001374 hasRelatedWork W2098979999 @default.
- W2364001374 hasRelatedWork W2107393972 @default.
- W2364001374 hasRelatedWork W2234827848 @default.
- W2364001374 hasRelatedWork W2349516295 @default.
- W2364001374 hasRelatedWork W2358158651 @default.
- W2364001374 hasRelatedWork W2360328028 @default.
- W2364001374 hasRelatedWork W2366531931 @default.
- W2364001374 hasRelatedWork W2378501032 @default.
- W2364001374 hasRelatedWork W2384996933 @default.
- W2364001374 hasRelatedWork W2386552498 @default.
- W2364001374 hasRelatedWork W2391083711 @default.
- W2364001374 hasRelatedWork W2662502976 @default.
- W2364001374 hasRelatedWork W279223347 @default.
- W2364001374 hasRelatedWork W2994563771 @default.
- W2364001374 hasRelatedWork W3091677456 @default.
- W2364001374 hasRelatedWork W2840463301 @default.
- W2364001374 isParatext "false" @default.
- W2364001374 isRetracted "false" @default.
- W2364001374 magId "2364001374" @default.
- W2364001374 workType "article" @default.