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- W2364002592 abstract "To prepare the polyclonal antibody against recombinant E2 protein of bovine viral diarrhea virus(BVDV) in rabbits,E.coli BL21(DE3) was transformed with the recombinant plasmid pET30a-E2.The recombinant E2 protein was expressed in E.coli after cultivation and induction.The purified recombinant E2 protein could be recognized by specific BVDV antisera in western blot.Then the purified recombinant E2 protein was used as antigen for immunizing rabbit to prepare polyclonal antibody against the recombinant E2 protein.The result of virus neutralization test showed that the titer of the polyclonal antibody to neutralize BVDV was 1:2,048.The polyclonal antibody against the recombinant E2 protein of BVDV also had highly reactivity and specialty in immunofluorescence analysis and western blot.The polyclonal antibody against recombinant E2 protein of BVDV developed in rabbits could be used in detection of BVDV in China and provided a good basis for establishing an ELISA for detecting of E2 protein of BVDV." @default.
- W2364002592 created "2016-06-24" @default.
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- W2364002592 date "2011-01-01" @default.
- W2364002592 modified "2023-09-23" @default.
- W2364002592 title "Preparation and characterization of antisera against recombinant E2 protein of bovine viral diarrhea virus" @default.
- W2364002592 hasPublicationYear "2011" @default.
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