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- W2364007669 abstract "The factors affecting the binding characteristics of GBSS with starch granule were studied using a wheat(Triticum aestivum) cultivar Chinese Spring with different temperature treatments.After sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) analysis,we got GBSS concentration by coomassie brilliant blue G-250(CBB G-250) method.The results showed that the temperature affected the binding of GBSS and starch granule.In 5080℃,the concentration of GBSS unbound from starch increased with the temperature rising,which the maximal concentration was 11.361 μg mL-1 at 80℃,while that was reduced when the temperature was 8595℃.Furthermore,Mg2+ could also affect the quantity of GBSS unbound from starch.When Mg2+ concentration was lower than 1.75 mmol L-1,the concentration of GBSS unbound increased.The lower Mg2+ concentration,the higher the concentration of GBSS unbound.However,when Mg2+ concentration was higher than 2.5 mmol L1,it could restrain GBSS unbinding.These results showed that GBSS bound starch granule by non-covalent bonds.The best GBSS extracting conditions were 55 mmol L-1 Tris-HCl(pH 6.8),0.75 mmol L-1 MgCl2, 2.3% SDS,5% 2-ME and 10% glycerol,15 min in boiling water,or 55 mmol L-1 Tris-HCl(pH 6.8),0.75 mmol L-1 MgCl2,2.3% SDS,5% 2-ME and 10% glycerol,80℃ 30 min.The results are helpful to investigate 3-D structure of biological activated GBSS and mechanism of GBSS binding with starch granule." @default.
- W2364007669 created "2016-06-24" @default.
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- W2364007669 date "2007-01-01" @default.
- W2364007669 modified "2023-09-22" @default.
- W2364007669 title "Binding Characteristics of Granule-Bound Starch Synthase(GBSS) with Starch in Wheat Cultivar Chinese Spring" @default.
- W2364007669 hasPublicationYear "2007" @default.
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