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- W2364015385 abstract "In this paper,the effects of different explants,the durations of pre-culture and co-culture on transformation frequency mediated by Agrobacterium were studied.The results showed that calli could be induced from both leaves and stems of potatoes and that optimized system was pre-cultured of explants for 2 days before transformation,infecting explants by Agrobacterium with 0.5 OD600 for 10 min,co-culture of explants and Agrobacterium for 3 days.A ribozyme gene targeting against two sites on negative strand of potato spindle tuber viroid(PSTVd) was transformed into potato by this optimized system." @default.
- W2364015385 created "2016-06-24" @default.
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- W2364015385 date "2013-01-01" @default.
- W2364015385 modified "2023-09-23" @default.
- W2364015385 title "Influencing Factors to Potato Transformation Mediated by Agrobacterium" @default.
- W2364015385 hasPublicationYear "2013" @default.
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