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- W2364017788 abstract "OBJECTIVE To construct anti-human AFP VH single domain antibody fusion protein gene,transform it into E.coli BL-21(DE3) for expression and identify its bioactivity.METHODS The VH single domain antibody gene was amplified by PCR from the vector constructed,and cloned into the prokaryotic fusion protein expression vector pET32a(+)and expressed in E.coli BL-21(DE3).The expression product was identified by SDS-PAGE,Western-blot,competitive inhibition ELISA and immunocytochemistry.RESULTS The VH single domain antibody gene consisted of 339 bp,which was cloned into an expression vector pET32a(+)and then transformed into E.coli BL-21(DE3).The pET32-VH vector producted a 33 kD fusion protein in E.coli BL-21(DE3) at a high level of total cellular protein,which was in the form of cytorrhyctes.After preliminary purification and renaturation,the VH single domain antibody fusion protein was obtained.Competitive inhibition ELISA and immunocytochemistry proved that the expression product has the special affinity with AFP.CONCLUSION Anti-AFP single domain antibody fusion protein has been successfully constructed and expressed for the use in clinical studies in future." @default.
- W2364017788 created "2016-06-24" @default.
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- W2364017788 date "2007-01-01" @default.
- W2364017788 modified "2023-09-23" @default.
- W2364017788 title "Construction,expression and identification of anti-human AFP V_H single domain antibody fusion protein gene" @default.
- W2364017788 hasPublicationYear "2007" @default.
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