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- W2364027244 abstract "Antigen gene VP7 of Human rotavirus was amplified by RT-PCR,and its 5′end and 3′end were respectively combined with the leading sequence and 3′-UTR of PVY.The modified gene was cloned into plant expression vector pBI121 containing CaMV35s.The plasmids recombined were transferred into Agrobacterium tumefaciens.Resistant plants of potato were produced using an Agrobacterium-mediated transformation system.Result showed that antigen genes were transmitted into potatoes and antigen VP7 has been expressed by PCR,PCR-Southern blot,Southern blot and ELISA assay.The expression system will be useful for producing oral vaccine in the future." @default.
- W2364027244 created "2016-06-24" @default.
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- W2364027244 date "2005-01-01" @default.
- W2364027244 modified "2023-09-25" @default.
- W2364027244 title "EXPRESSION OF ANTIGEN GENE VP7 FROM G3 STRAIN OF ROTAVIRUS IN POTATO" @default.
- W2364027244 hasPublicationYear "2005" @default.
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