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- W2364037149 abstract "Plackett-Burman design was used to evaluate importance of the selected fifteen internal and external factors to the growth of Ochrobactrum intermedium DN2. Results showed that tryptone, MgSO_4·7H_2O, temperature, the initial pH value, volume of medium and incubation time were the main factors affecting the growth of strain DN2. In addition, the central composite design and response surface analysis were used to determine the optimal levels of the main factors. The optimized medium was determined as follows: tryptone 11.34g/L,beef extract 3.00g/L,NaCl 5.00g/L, MgSO_4·7H_2O 3.71g/L,pH 7.23. The optimal cultural conditions were cultural temperature, 32℃;rotatory speed,120r/min ;volume of medium,88ml/250ml Erlenmeyer and incubation time,34h. Under these conditions, cell density of strain DN2 can increase from 7.35×108cfu/ml to 6.49×109cfu/ml and time used to obtain maximal cell production decreases from 48h to 36h. The experimental data have validated the theoretical values." @default.
- W2364037149 created "2016-06-24" @default.
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- W2364037149 date "2005-01-01" @default.
- W2364037149 modified "2023-09-26" @default.
- W2364037149 title "Optimization of Culture Medium Components and the Culture Conditions of O. intermedium DN2 for Growth by Response Surface Methodology" @default.
- W2364037149 hasPublicationYear "2005" @default.
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