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- W2364037286 abstract "OBJECTIVE:To investigate the influence of CXCR4-PI3KⅢ-autophagy to migration and invasion of LoVo cell.METHODS:The LoVo cells were grouped to normal LoVo cell group,CXCR4-RNAi(mediated with lentivirus)group,and nonsence control group(NC).PI3K Ⅲ mRNA expression level was measured with Q-PCR.Western blot was employed to measure the expression of PI3K Ⅲ,LC3-Ⅱ,and Beclin 1protein.Laser canning confocal microscope was used to identify the green fluorescence particles.Transmission electron microscope was employed to observe autolysosome.Transwell chamber was applied to evaluate the migration and invasion of LoVo cell.RESULTS:The PI3K Ⅲ mRNA expression level was 1.09±0.11in normal LoVo cell group,1.07±0.25in NC group,and 0.86±0.06in CXCR4-RNAi group.The relative expression level of PI3K Ⅲ was significantly lower in CXCR4-RNAi group(0.227±0.023)than that in normal LoVo cell group(0.607±0.012,P0.001)and in NC group(0.667±0.040,P0.001),and there was no significant difference between normal LoVo cell group and NC group(P=0.310).The relative expression level of LC3-Ⅱ was significantly lower in CXCR4-RNAi group(0.083±0.012)than that in normal LoVo cell group(0.233±0.015,P0.001)and in NC group(0.253±0.020,P0.001),there was no significant difference between normal LoVo cell group and NC group(P=0.184).The relative expression level of Beclin 1was 0.207±0.012in CXCR4-RNAi group,0.440±0.053(P=0.134)in normal LoVo cell group,and 0.650±0.252(P=0.011)in NC group.The relative expression level of PI3K Ⅲ,LC3-Ⅱ,and Beclin 1was significantly lower in CXCR4-RNAi group than that in normal LoVo cell group and in NC group.There was more green fluorescence particles and autolysosomes in normal LoVo cell group and in NC group than that in CXCR4-RNAi group.The migration experiment showed the mean number of LoVo cell in CXCR4-RNAi group(87.7±9.1)was lower than that in normal LoVo cell group(126.3±4.9,P=0.009)and in NC group(115.0±18.7,P=0.035).The invasion experiment found the mean number of LoVo cell in CXCR4-RNAi group(88.7±10.5)was lower than that in normal LoVo cell group(109.7±4.7,P=0.019)and in NC group(107.7±8.1,P=0.029).CONCLUSION:CXCR4-PI3KⅢ-autophagy axis can regulate the cell autophagy level,influence the migration and invasion power of LoVo cell,and it may be one of the mechanisms of invasion and metastasis of malignant tumors." @default.
- W2364037286 created "2016-06-24" @default.
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- W2364037286 date "2013-01-01" @default.
- W2364037286 modified "2023-09-23" @default.
- W2364037286 title "Relationship of CXCR4-PI3K III-autophagy axis and LoVo cell migration and invasion" @default.
- W2364037286 hasPublicationYear "2013" @default.
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