Matches in SemOpenAlex for { <https://semopenalex.org/work/W2364037322> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2364037322 abstract "OBJECTIVE:To investigate the anti-proliferation and apoptosis inducing effects of ponicidin (PON) on leukemic NB4 cells and its mechanisms of action.METHODS:NB4 cells in culture medium in vitro were given with different concentrations of PON (0-50 μmol/L) for 0,24,48 and 72 h.The inhibitory rate of the cells was measured by MTT assay and cell apoptosis was examined by flow cytometry (FCM).Western blotting was used to detect Caspase-3 and poly(ADP-ribose) polymerase (PARP) expression as well as apoptotic modulators such as Bax,Bcl-2,Bak,Bad and Survivin.RESULTS:PON (over 20 μmol/L) inhibited the growth of NB4 cells in both time-and dose-dependent manners.The cell apoptosis was demonstrated obviously by FCM and marked morphological changes of cell apoptosis such as the condensation of chromatin was clearly observed by Hoechst staining after treatment for 48 h.Western blotting showed cleavage of the Caspase-3 zymogen protein,with the appearance of its 17×103 subunit,and a cleaved 89×103 fragment of PARP was also found.Furthermore,Western blotting showed that anti-apoptotic protein Survivin decreased dramatically while pro-apoptotic protein Bax increased significantly after the cells were treated by PON for 48 h.CONCLUSIONS:PON exhibits in vitro anti-leukemia effect by induction of apoptosis in NB4 cells,and downregulation of Survivin as well as upregulation of Bax expression may be one of its significant apoptosis inducing mechanism." @default.
- W2364037322 created "2016-06-24" @default.
- W2364037322 creator A5028313744 @default.
- W2364037322 date "2010-01-01" @default.
- W2364037322 modified "2023-09-28" @default.
- W2364037322 title "Investigation of apoptosis inducing effect of ponicidin on leukemia NB4 cells in vitro" @default.
- W2364037322 hasPublicationYear "2010" @default.
- W2364037322 type Work @default.
- W2364037322 sameAs 2364037322 @default.
- W2364037322 citedByCount "0" @default.
- W2364037322 crossrefType "journal-article" @default.
- W2364037322 hasAuthorship W2364037322A5028313744 @default.
- W2364037322 hasConcept C104317684 @default.
- W2364037322 hasConcept C12519072 @default.
- W2364037322 hasConcept C127561419 @default.
- W2364037322 hasConcept C153911025 @default.
- W2364037322 hasConcept C182979987 @default.
- W2364037322 hasConcept C185592680 @default.
- W2364037322 hasConcept C190283241 @default.
- W2364037322 hasConcept C202751555 @default.
- W2364037322 hasConcept C2775975398 @default.
- W2364037322 hasConcept C2776415932 @default.
- W2364037322 hasConcept C2780783641 @default.
- W2364037322 hasConcept C31573885 @default.
- W2364037322 hasConcept C552990157 @default.
- W2364037322 hasConcept C553184892 @default.
- W2364037322 hasConcept C55493867 @default.
- W2364037322 hasConcept C82381507 @default.
- W2364037322 hasConcept C85265743 @default.
- W2364037322 hasConcept C86803240 @default.
- W2364037322 hasConceptScore W2364037322C104317684 @default.
- W2364037322 hasConceptScore W2364037322C12519072 @default.
- W2364037322 hasConceptScore W2364037322C127561419 @default.
- W2364037322 hasConceptScore W2364037322C153911025 @default.
- W2364037322 hasConceptScore W2364037322C182979987 @default.
- W2364037322 hasConceptScore W2364037322C185592680 @default.
- W2364037322 hasConceptScore W2364037322C190283241 @default.
- W2364037322 hasConceptScore W2364037322C202751555 @default.
- W2364037322 hasConceptScore W2364037322C2775975398 @default.
- W2364037322 hasConceptScore W2364037322C2776415932 @default.
- W2364037322 hasConceptScore W2364037322C2780783641 @default.
- W2364037322 hasConceptScore W2364037322C31573885 @default.
- W2364037322 hasConceptScore W2364037322C552990157 @default.
- W2364037322 hasConceptScore W2364037322C553184892 @default.
- W2364037322 hasConceptScore W2364037322C55493867 @default.
- W2364037322 hasConceptScore W2364037322C82381507 @default.
- W2364037322 hasConceptScore W2364037322C85265743 @default.
- W2364037322 hasConceptScore W2364037322C86803240 @default.
- W2364037322 hasLocation W23640373221 @default.
- W2364037322 hasOpenAccess W2364037322 @default.
- W2364037322 hasPrimaryLocation W23640373221 @default.
- W2364037322 hasRelatedWork W1997953193 @default.
- W2364037322 hasRelatedWork W2054361489 @default.
- W2364037322 hasRelatedWork W2351206228 @default.
- W2364037322 hasRelatedWork W2352170594 @default.
- W2364037322 hasRelatedWork W2359026939 @default.
- W2364037322 hasRelatedWork W2369565816 @default.
- W2364037322 hasRelatedWork W2372638622 @default.
- W2364037322 hasRelatedWork W2378147551 @default.
- W2364037322 hasRelatedWork W2379149892 @default.
- W2364037322 hasRelatedWork W2379255386 @default.
- W2364037322 hasRelatedWork W2379983780 @default.
- W2364037322 hasRelatedWork W2388809393 @default.
- W2364037322 hasRelatedWork W2392942585 @default.
- W2364037322 hasRelatedWork W2393048338 @default.
- W2364037322 hasRelatedWork W2393309360 @default.
- W2364037322 hasRelatedWork W2393870434 @default.
- W2364037322 hasRelatedWork W2413212649 @default.
- W2364037322 hasRelatedWork W2789310883 @default.
- W2364037322 hasRelatedWork W3025269802 @default.
- W2364037322 hasRelatedWork W3025547952 @default.
- W2364037322 isParatext "false" @default.
- W2364037322 isRetracted "false" @default.
- W2364037322 magId "2364037322" @default.
- W2364037322 workType "article" @default.