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- W2364037887 abstract "To study whether can increase the effect of freeze drying when human red blood cells(RBCs) were incubated in trehalose loading buffer adding low concentration dimethylsulfoxide(DMSO) before lyophilization.The samples were divided into two groups : the control group(loading buffer was PBS),and the test group(adding 6% DMSO to the 800 mmol/Ltrehalose loading solution).After incubated at 37℃ for 8 hours and freeze dried in program,we used 15% PVP solution rehydrated.Compared two group RBCs with hemoglobin recovery,RCD,the stability of rehydrated RBCs when stored in 4℃ and long-term preservation of lyophilized RBCs.The results showed that the rehydrated-lyophilized RBCs of tese group which hemoglobin recovery was(63.019±4.901)% and control group was(28.787±7.514)%(P0.01);that RCD of rehydrated-lyophilized RBCs decreased obviously which of test group was(18.133±2.392)% but control group was(8.017±1.934)%(P0.01).After rehydrated-lyophilized RBCs stored in 4℃,contrast to the baseline hemoglobin recovery of 4 h,6 h and 8 h had statistical difference between two groups(P0.05) but which of 12 h and 24 h showed no difference.Contrast to the baseline hemoglobin recovery of lyophilized RBCs stored for 6 month in 4℃ had statistical difference between two groups(P0.05).It is concluded that after handled with trehalose-loading buffer adding 6% DMSO,rehydrated-lyophilized human RBCs can increase hemoglobin recovery of RBCs,improve RCD,enhance the stability of rehydrated RBCs when stored in 4℃ refrigerate and long-term preservation of lyophilized RBCs." @default.
- W2364037887 created "2016-06-24" @default.
- W2364037887 creator A5047909116 @default.
- W2364037887 date "2008-01-01" @default.
- W2364037887 modified "2023-09-25" @default.
- W2364037887 title "Study of Freeze Drying of Human RBCs Loaded Trehalose and DMSO" @default.
- W2364037887 hasPublicationYear "2008" @default.
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