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- W2364043432 abstract "Objective: To construct secretion eukaryotic expression vector pSecTag-EGFP and study the transfection efficiency in chicken embryonic fibroblast cells,in establishing the foundation for the aim gene expression in chicken embryonic fibroblast cells.Methods: EGFP gene was cloned and secretion eukaryotic expression vector was constructed by the method of digestion ligation and transformation.An optimum experiment was designed and chicken embryonic fibroblast cells with pSecTag-EGFP was transfectted.The things of the EGFP gene expression was observed under fluorescence microscope.Results: It was efficient to transfect chicken embryonic fibroblast cells by the method of lipofectamine mediation.The expression levels of protein were significantly different.The optimum condition of transfection was with 2μl lipofectamine and 1μg expression vector in medium.Conclusion: pSecTag-EGFP eukaryotic expression vectoris constructed successfully,and an optimum condition of transfection is found." @default.
- W2364043432 created "2016-06-24" @default.
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- W2364043432 date "2007-01-01" @default.
- W2364043432 modified "2023-09-23" @default.
- W2364043432 title "Construction of eukaryotic expression vector pSecTag-EGFP and the study of transfecting chicken embryonic fibroblast cells" @default.
- W2364043432 hasPublicationYear "2007" @default.
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