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- W2364079365 abstract "Objective: To establish a mouse hepatocellular carcinoma cell line that can produce mMIP 1α and to evaluate the possibility of cancer gene therapy by mMIP 1α. Methods: mMIP 1α cDNA was cloned into retrovirus vector pBabe puro and pBabe puro mMIP 1α was constructed, then pBabe puro mMIP 1α was used to transfect packaging cells, anti puromycin cells was proliferated, the supernatant was used to infect hepa1 6, the anti puromycin clone (hepa1 6 mMIP 1α) and hepa1 6 were analysed for the expression of mMIP 1α mRNA and protein by RT PCR and immunohistochemistry respectively. The growth curve of hepa1 6 and hepa1 6 mMIP 1α was drawn. The chemotaxis of mMIP 1α produced by hepa1 6 mMIP 1α to mouse spleen cells was observed on agarose gel. C57B/L mouse was inoculated with the tumor cell and the tumorigenicity was studied. Results: Recombinant retrovirus vector pBabe puro mMIP 1α with mMIP 1α cDNA was constructed. Hepa1 6 did not produce mMIP 1α mRNA and protein, while hepa1 6 mMIP 1α could produce mMIP 1α mRNA and protein. The growth curve of hepa1 6 and hepa1 6 mMIP 1α showed no difference. The chemotaxis of mMIP 1α produced by hepa1 6 mMIP 1α to mouse spleen cells was observed. The tumorigenicity was reduced. Conclusion: A mouse hepatocellular carcinoma Hepa1 6 mMIP 1α is established and mMIP 1α can affect the tumorigenecity of hepa1 6." @default.
- W2364079365 created "2016-06-24" @default.
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- W2364079365 date "2001-01-01" @default.
- W2364079365 modified "2023-09-25" @default.
- W2364079365 title "Establishment of a mouse hepatocellular carcinoma cell line producing mMIP-1α chemokines and the tomorigenicity of mMIP-1α transfected Hepa1-6" @default.
- W2364079365 hasPublicationYear "2001" @default.
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