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- W2364096995 abstract "To establish human lactoferrin cDNA transgenic donor cell lines and prepare competent donor cells for the production of transgenic animals by somatic cell nuclear transfer (SCNT) and detect the tissue-specific expression of human lactoferrin cDNA, Human lactoferrin cDNA was obtained by RT-PCR while 6.5 kb caprine(Capra hircus) β-casein regulatory elements were amplified by Long and Acute PCR. Subsequently the two fragments were inserted into the corresponding site of the plasmid pEGFP-C1, resulting in the construction of mammary specific expression vector p6.5hLF-EGFP. Then the caprine fetal fibroblast cells were transfected with p6.5hLF-EGFP by LipofectamineTM-2000 and selected with G418 for 3 to 4 weeks. The G418 resistant transfectants were identified by PCR and EGFP detection. The results indicated that the transgene was stably integrated into the open region of the chromatin of G418 resistant fibroblast cells. The expression of human lactoferrin was identified in the supernant of stable transfected mammary epithelial cells by Western blot. This study may provide competent transgenic donor cells for the production of transgenic animals by SCNT and improve the efficiency of transgenic cloning." @default.
- W2364096995 created "2016-06-24" @default.
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- W2364096995 date "2008-01-01" @default.
- W2364096995 modified "2023-09-28" @default.
- W2364096995 title "Establishment of Human Lactoferrin cDNA Transgenic Caprine Fetal Fibroblast Cell Lines" @default.
- W2364096995 hasPublicationYear "2008" @default.
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