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- W2364106319 abstract "After enzymatic lysis and ultrasonication, Corynebacterium glutamicum, which produces high yield of glutamine, can release glutamine synthetase (GS) with high activity. The GS extracellular catalysis is regulated by a few conditions and factors. By mono-factor experiment, response experiment and orthogonal experiment, the suitable conditions for GS extracellular catalysis are determined: in the reaction mixture, the most significant factor is ATP, and the concentration of ATP is 40~50 mmol/L. The amount of enzyme also shows strong effect to the reaction. Using the cell-free extracts, the effects of some factors on the GS conversion in the extracellular reaction are examined and the optimal condition for GS extracellular catalysis obtained. The highest conversion is 7.1 g/L and the conversion yield is 94.8%." @default.
- W2364106319 created "2016-06-24" @default.
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- W2364106319 date "2002-01-01" @default.
- W2364106319 modified "2023-09-25" @default.
- W2364106319 title "Glutamine Production by the Enzyme Method" @default.
- W2364106319 hasPublicationYear "2002" @default.
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