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- W2364111982 abstract "Root rot of Gerbera jamesonii caused by Phytophthora cryptogea is a serious disease in China, especially in wet condition.Since many pathogens were reported could infect the root of G.jamesonii and the isolation and identification of Phytophthora is really difficult,a rapid and accurate method for specific detection of P.cryptogea is necessary for determination of root rot in diseased G.jamesonii tissue.Based on the alignment of ITS sequences from Oomycetes,two primers were synthesized and a PCR based protocol was designed to detect and diagnostic of the G.jamesonii root rot caused by P.cryptogea specifically. Among 46 isolates representing 23 species of Oomycetes and fungi,a PCR product about 620 bp could only be amplified from P.cryptogea.The detection sensitivity is about 10 pg.The PC primer based PCR assay will provide a accurate and sensitivity tool for detection of P.cryptogea in G.jamesonii root rot." @default.
- W2364111982 created "2016-06-24" @default.
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- W2364111982 date "2006-01-01" @default.
- W2364111982 modified "2023-09-25" @default.
- W2364111982 title "Rapid diagnostic of Phytophthora Root rot of Gerbera jamesonii by PCR" @default.
- W2364111982 hasPublicationYear "2006" @default.
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