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- W2364112712 abstract "To study bacterial diversity of shrimp pond sediment and improve culture technique,8 kinds of media were used to isolate bacteria from shrimp pond sediment.Bacterial colonies were collected and denaturing gradient gel electrophoresis(DGGE) technique was used to study the bacterial community structure and it was compared with that of in situ sample.Most of bacteria appeared in the first 2 weeks.During the 3rd week and the 12 th week,there were some new isolates on VL55,DNB,HM2 and DR2A plates,and the colony number was no obviously increasing on other plates.Agar and gellan gum were used as gelling agent separately,the culture results showed that gellan gum plates had a high moisture retention,more bacterial number and high bacterial diversity than that on agar plates.By the analysis of DGGE profile,there were about 60 kinds of bacterial strains in the sediment.Cultured by the media,about 50 kinds of bacterial strains were detected.The number of it was up to 83% of that of the sediment.However,the bacterial community structure had a great variation and the dominant microorganisms changed.It was confirmed by sequencing results too.There were high bacterial diversity in the shrimp pond sediment.Improvement of medium,using of gellan gum and prolongation of culture time effectively obtained the culturability of the shrimp pond sediment." @default.
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- W2364112712 date "2010-01-01" @default.
- W2364112712 modified "2023-09-26" @default.
- W2364112712 title "Bacterial Diveisity in Aquaculture Environment and Optimization of Culturing Technique" @default.
- W2364112712 hasPublicationYear "2010" @default.
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