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- W2364123030 abstract "Objective To explore the molecular mechanism of glioma cell apoptosis and improve the effect of chemotherapy for glioma.Methods pLXSN-IL2-TNFα was introduced into packaging cell line PA317 by lipofectmine transfection method.Several G418-resistant colonies were obtained and the virus titer in supernatant of the colonies was determined by NIH3T3 cells with the highest one of 5×10+5CFU/ml.C6 rat glioma cell line was exposed in vitro to pLXSN-IL2-TNFα.The biological activity of IL2 in culture supernatant was monitored in the range of 2.0～8.0U/10+6 cells/24 h while that of TNFα was in 23～110U/10+6 cells/24 h.Cell apoptosis was ascertained by (1) cell morphology;(2)DNA fragment analysis;(3)fluoroscopy;(4)flow cytometric study.Results When C6 glioma cells were treated with IL2-TNFα for 72 hours,cell apoptosis occurred.Typical apoptotic morphological features of cell shrinkage and condensation and margination of nuclear chromatin were showed by light and electron microscopy.Condensed nuclear chromatin and the fragments of nuclei were demonstrated by fluoroscopy.Apoptotic peak was identified by blow cytometric study.The apoptotic cells accounted for (14.5±1.3)% of the cell population.DNA ladder was not showed by DNA fragment analysis.Conclusion The apoptosis of C6 glioma cell was successsfully induced by IL2-TNFα.;" @default.
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- W2364123030 title "Induction of Apoptosis by IL2-TNFα in C6 Rat Glioma" @default.
- W2364123030 hasPublicationYear "2001" @default.
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