Matches in SemOpenAlex for { <https://semopenalex.org/work/W236413090> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W236413090 endingPage "653" @default.
- W236413090 startingPage "646" @default.
- W236413090 abstract "The microsomal enzyme epoxide hydratase has been purified to homogeneity as judged by electrophoretical, ultracentrifugal and immunological criteria and by C- and N-terminal analysis. The preparation procedure consisted of solubilisation using the non-ionic detergent cutscum, (NH4)2SO4 precipitation, ion-exchange chromatography on DEAE-cellulose and cellulose phosphate and hydrophobic chromatography on butyl-sepharose. The product was detergent-free, had a relatively high content of hydrophobic amino acids and tended to aggregate in aqueous solutions. The protein had a minimum molecular weight of 49,000 ± 500 with a sedimentation coefficient of S20w≃ 3. Antibodies raised against the homogeneous preparation precipitated the entire hydratase activity in solubilised microsomes towards benzo(a)pyrene 4,5-(K-region-)oxide and styrene oxide as substrates. Moreover, the pure enzyme had a very broad substrate specificity, hydrating both arene and alkene oxides. The same general relationship between the hydration velocities of 6 K-region epoxides of polycyclic hydrocarbons was obtained with rat liver microsomal fractions and pure epoxide hydratase. These findings suggest that the enzyme isolated is the only one present in the rat liver microsomal fractions which is responsible for the hydration of such substrates." @default.
- W236413090 created "2016-06-24" @default.
- W236413090 creator A5021366830 @default.
- W236413090 creator A5046143290 @default.
- W236413090 date "1977-01-01" @default.
- W236413090 modified "2023-10-03" @default.
- W236413090 title "ISOLATION OF RAT LIVER EPOXIDE HYDRATASE: PROPERTIES AND SUBSTRATE SPECIFICITY OF THE PURE ENZYME" @default.
- W236413090 cites W1559790796 @default.
- W236413090 cites W1974648730 @default.
- W236413090 cites W1975773212 @default.
- W236413090 cites W1984713897 @default.
- W236413090 cites W1989043310 @default.
- W236413090 cites W1990580720 @default.
- W236413090 cites W1999070156 @default.
- W236413090 cites W2011176046 @default.
- W236413090 cites W2027284532 @default.
- W236413090 cites W2038069616 @default.
- W236413090 cites W2048307591 @default.
- W236413090 cites W2054107475 @default.
- W236413090 cites W2071726083 @default.
- W236413090 cites W2082173903 @default.
- W236413090 cites W2090530265 @default.
- W236413090 cites W2156389002 @default.
- W236413090 doi "https://doi.org/10.1016/b978-0-08-021523-5.50089-4" @default.
- W236413090 hasPublicationYear "1977" @default.
- W236413090 type Work @default.
- W236413090 sameAs 236413090 @default.
- W236413090 citedByCount "0" @default.
- W236413090 crossrefType "book-chapter" @default.
- W236413090 hasAuthorship W236413090A5021366830 @default.
- W236413090 hasAuthorship W236413090A5046143290 @default.
- W236413090 hasConcept C1388644 @default.
- W236413090 hasConcept C15920480 @default.
- W236413090 hasConcept C161790260 @default.
- W236413090 hasConcept C178790620 @default.
- W236413090 hasConcept C181199279 @default.
- W236413090 hasConcept C185592680 @default.
- W236413090 hasConcept C18903297 @default.
- W236413090 hasConcept C2776186722 @default.
- W236413090 hasConcept C2776920199 @default.
- W236413090 hasConcept C2777289219 @default.
- W236413090 hasConcept C2777566558 @default.
- W236413090 hasConcept C43617362 @default.
- W236413090 hasConcept C521977710 @default.
- W236413090 hasConcept C55493867 @default.
- W236413090 hasConcept C86803240 @default.
- W236413090 hasConcept C87644729 @default.
- W236413090 hasConceptScore W236413090C1388644 @default.
- W236413090 hasConceptScore W236413090C15920480 @default.
- W236413090 hasConceptScore W236413090C161790260 @default.
- W236413090 hasConceptScore W236413090C178790620 @default.
- W236413090 hasConceptScore W236413090C181199279 @default.
- W236413090 hasConceptScore W236413090C185592680 @default.
- W236413090 hasConceptScore W236413090C18903297 @default.
- W236413090 hasConceptScore W236413090C2776186722 @default.
- W236413090 hasConceptScore W236413090C2776920199 @default.
- W236413090 hasConceptScore W236413090C2777289219 @default.
- W236413090 hasConceptScore W236413090C2777566558 @default.
- W236413090 hasConceptScore W236413090C43617362 @default.
- W236413090 hasConceptScore W236413090C521977710 @default.
- W236413090 hasConceptScore W236413090C55493867 @default.
- W236413090 hasConceptScore W236413090C86803240 @default.
- W236413090 hasConceptScore W236413090C87644729 @default.
- W236413090 hasLocation W2364130901 @default.
- W236413090 hasOpenAccess W236413090 @default.
- W236413090 hasPrimaryLocation W2364130901 @default.
- W236413090 hasRelatedWork W1978629203 @default.
- W236413090 hasRelatedWork W2010372900 @default.
- W236413090 hasRelatedWork W2029815989 @default.
- W236413090 hasRelatedWork W2052034756 @default.
- W236413090 hasRelatedWork W2073513478 @default.
- W236413090 hasRelatedWork W2100783303 @default.
- W236413090 hasRelatedWork W2326549699 @default.
- W236413090 hasRelatedWork W2362970860 @default.
- W236413090 hasRelatedWork W2414307502 @default.
- W236413090 hasRelatedWork W975261258 @default.
- W236413090 isParatext "false" @default.
- W236413090 isRetracted "false" @default.
- W236413090 magId "236413090" @default.
- W236413090 workType "book-chapter" @default.