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- W2364135763 abstract "[Objective]In order to provide references for the reasonable use of L. plantarum ST-Ⅲ, rapid qualitative and quantitative methods for ST-Ⅲ detection in probiotics products were established through this research.[Method]The differences in the whole genome sequences between ST-Ⅲ and the other three Lactobacillus plantarum strains accessed from the NCBI database were compared and the strain specificity primers of ST-Ⅲ was designed accordingly in order to carry out strain-specific PCR analysis and fluorescent quantitative real-time PCR detection. The established real-time PCR method was used to detect the quantity of ST-Ⅲ in probiotics products. [Result]The designed primers had good specificity for ST-Ⅲ strain. The size of the expected specific band presented in the ST-Ⅲ samples using PCR amplification was similar to that of the aiming band. The curve of established fluorescent quantitative real-time PCR was y= -3.369lgx+39.84(R2=0.9990), which could demand the usage of real-time PCR, and its detection limit was 2.85×103 CFU/mL. By this detection method, the quantity of ST-Ⅲ in probiotics products was 3.25×105, 1.28×106, and 4.55×106 CFU/mL, respectively. [Conclusion]The established methods for ST-Ⅲ detection were rapid, accurate, and time-saving, therefore it could be used in relevant production processes." @default.
- W2364135763 created "2016-06-24" @default.
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- W2364135763 date "2012-01-01" @default.
- W2364135763 modified "2023-09-25" @default.
- W2364135763 title "Development on rapid qualitative and quantitative detection methods for Lactobacillus plantarum ST-III in probiotics products" @default.
- W2364135763 hasPublicationYear "2012" @default.
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