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- W2364140409 abstract "Objective:To clone the catalytic region of Glucosyltransferase from Streptococcus mutans GS5 and express it in Escherichia coli BL21 by IPTG induction,obtain the soluble rGTF and activity assay. Method:PCR and construction of fusion protein technique,IPTG induction,anthrone-sulfuric acid assay. Result:The CAT gene was cloned correctly and its fusion protein was expressed in E.coli by IPTG induction. The SDS-PAGE results showed that the rGTF is soluble,and anthrone-sulfuric acid assay demonstrated its biological activity. Conclusion:The aimed gene and its fusion protein were gained successfully,and to be demonstrated its biological activity which provides a base to the further research." @default.
- W2364140409 created "2016-06-24" @default.
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- W2364140409 date "2010-01-01" @default.
- W2364140409 modified "2023-09-23" @default.
- W2364140409 title "IPTG induction of the soluble catalytic region of S.mutans expression and activity assay" @default.
- W2364140409 hasPublicationYear "2010" @default.
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