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- W2364152624 abstract "We reported the nucleotide and encoded amino acid sequences of the envelope (E) protein gene of a dengue-2(DEN-2) virus strain 04, isolated from a patient in Hainan, China in 1985. The sequences were compared with the corresponding sequences from DEN-1, DEN-3, DEN-4 and other DEN-2 viruses: the prototype New Guinea G virus (NGG), the Jamaican (JAM) strain 1409, the candidate vaccine strain PR159 (S1) from Puerto Rico and three Malaysia isolates (M1, M2 and M3). The nucleotide sequence encoding the E protein was determined by dideoxyribonucleotide chain termination method. Comparison of D_2-04 E nucleotide sequence with the corresponding sequence from NGC, JAM and S1 revealed similarities of 95.0%, 97.4% and 90.0% respectively. The other DEN-2 viruses showed similarities from 92.3% (M1) to 94.3% (M3), while the other dengue serotypes showed similarities of about 65%. Comparison of the deduced amino acid sequence of D_2-04 E protein with the corresponding sequence from NGC, JAM, S1, M1, M2 and M3 revealed similarities of 96.8%, 97.2%, 96.4%, 95.0%, 95.6% and 94.3% respectively. The similarities with other dengue serotypes were 62—68%, while those with other flaviviruses ranged from 43.8% (yellow fever virus) to 47% (JE). The deduced amino acid sequence revealed seven cysteine residues conserved within the DEN 2 serotype, as well as two potential glycosylation sites at Asn-67 and Asn-153." @default.
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- W2364152624 date "1991-01-01" @default.
- W2364152624 modified "2023-09-23" @default.
- W2364152624 title "NUCLEOTIDE AND ENCODED AMINO ACID SEQUENCES OF THE ENVELOPE PROTEIN GENE OF A DENGUE-2 VIRUS ISOLATED IN CHINA" @default.
- W2364152624 hasPublicationYear "1991" @default.
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