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- W2364154173 abstract "Objective To determine whether quantitative reverse transcriptase polymerase chain reaction (RT PCR) with SYBR GREENⅠ fluorescence is a reliable and flexible method for detecting the gene expression for those rarely available tissues. Methods RNA was isolated from kidneys of 24 month old rats(6) and 3 mouth old rats(6). The level of the PAI 1(plasminogen activator inhibitor I) gene expression was measured using real time RT PCR with SYBR GREENⅠ. Glyceraldehyde phosphate dehydrogenase (G3PDH) was used as external control. Results Standard curves were linear and showed high correlations (R 2 0 99). Ratio of PAI 1 gene copies to G3PDH gene copies was calculated according to specific PCR products. The PAI 1 gene expression in kidneys of aged rats was significantly increased compared to that of the young rats 〔(1 27±0 90)×10 -3 vs (3 84±2 00)× 10 -3 , P 0 05). Conclusions If PCR conditions are controlled strictly, quantitative PCR with SYBR GREENⅠ fluorescence can be a reliable method for those rarely available tissues to detect the gene expression. The level of PAI 1 expression is higher in kidneys of the aged rats than that of young rats." @default.
- W2364154173 created "2016-06-24" @default.
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- W2364154173 date "2003-01-01" @default.
- W2364154173 modified "2023-09-23" @default.
- W2364154173 title "Quantitative RT-PCR for the expression of plasminogen activator inhibitor 1(PAI-1) in kidneys of aged rats" @default.
- W2364154173 hasPublicationYear "2003" @default.
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