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- W2364154813 abstract "Objective:To gain large amount of recombinant allergen Cla h8 of Cladosporium herbarum through genetic engineering method,which not only facilitates the standardization of allergen vaccination,but also is more suitable for diagnosis and treatment.Methods:The total RNA was acquired from C.herbarum culture,then the cla h8 gene fragments amplified by RT-PCR was cloned into vector pET-19b and then transformed to E.coli BL21 Star(DE3)pLysS.After induction,Cla h8 was expressed as inclusion body in E.coli BL21 Star(DE3)pLysS.The soluble protein,following purification and renaturation,was obtained.The immunological activity was identified by Dot-blot and Western blot.Results:The IgE and IgG of the serum from C.herbarum allergic patients could specially react with the recombinant Cla h8(rCla h8),and the immunological activity of rCla h8 was comparable with the native protein.Conclusion:We have successfully obtained the recombinant Cla h8 equipped with immunological activity,which leads to significant improvements in allergy diagnosis and treatment.The approach has also resolved the problems of natural allergen extracts associated with multi-component and difficult standardization." @default.
- W2364154813 created "2016-06-24" @default.
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- W2364154813 date "2011-01-01" @default.
- W2364154813 modified "2023-09-25" @default.
- W2364154813 title "Production of recombinant Cladosporium herbarum allergen Cla h8 and investigation on its immunological activities" @default.
- W2364154813 hasPublicationYear "2011" @default.
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