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- W2364155324 abstract "Objectives To use quantitative real-time PCR to detect the level of expression of the genes fbpB,psts1,and mpt64 coding for proteins in 66 strains of Mycobacterium tuberculosis and to explore variability in protein antigens of the Beijing genotype and non-Beijing genotype at the transcriptional level in drug-resistant strains and sensitive strains.Methods Specific primers were designed in accordance with the GenBank database.The amplified PCR fragments were cloned into respective vectors,and recombinant plasmids were purified and serially diluted.Levels of gene expression were detected using qRT-PCR.Results There was a statistically significant difference(t=3.721,P0.01)in the expression of psts1 in the Beijing genotype compared to the non-Beijing genotype.There was a statistically significant difference(t=3.951,P0.01)in the expression of mpt64 in drug-resistant strains compared to sensitive strains.The amplification curve for live M.tuberculosis was a characteristic S curve.The curve for dead M.tuberculosis and nontuberculous mycobacteria was a flat line;a fluorescent signal was not detected.Conclusion This study found that the expression of psts1 in the Beijing genotype is down-regulated compared to the non-Beijing genotype and the expression of mpt64 in drug-resistant strains is down-regulated compared to sensitive strains." @default.
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- W2364155324 date "2012-01-01" @default.
- W2364155324 modified "2023-09-24" @default.
- W2364155324 title "Level of expression of the genes coding for proteins in Mycobacterium tuberculosis according to qRT-PCR" @default.
- W2364155324 hasPublicationYear "2012" @default.
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