Matches in SemOpenAlex for { <https://semopenalex.org/work/W2364163722> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2364163722 abstract "Objective To study the characteristics of the binding of 125 I-VIP with VIP receptor in H22 hepatocarcinoma cells in mice and in vitro. Methods VIP was radio-labelled with carrier free 125 I with the chloramine T method and purified with sephadex G-50 column chromatography. Experiments of saturation binding and competition binding were carried to study the characteristics of the binding between 125 IVIP and the VIP receptors in the H22 cells in vitro. Experiments of dynamic biodistribution and competion distribution were performed to verify the characteristics of the binding between 125 I-VIP and VIP receptors in mice with H22 hepatocarcarcinoma. Results The labelling rate of 125 I to VIP was 73.8%. The specific radioactivity of 125 I-VIP was 18.2 TBq/mmol and the radiochemical purity was 98%. The binding of 125 I-VIP with VIP receptors in H22 cells was inhibited by non-labelled VIP in a dose-dependent manner. Two classes of specific high affinity binding sites for VIP expression in H22 cells were detected with scatchard analysis. The Kd was 1.74 nmol/L and 28.63 nmol/L and Bmax was 0.27 pmol/10 6 cells and 5.57 pmol/10 6 cells for high and low affinity binding sites respectively. Radioactivity accumulation was higher in the carcinoma tissue than in the muscles of the mice bearing H22 hepatocarcinoma in each phase (P0.05, P0.001). The maximal ratio of the carcinoma (T)/muscle (NT) was 2.68 at 20 min p.i. The radioactivity was higher in the lungs than in the blood and the liver (P0.05, P0.01). The radioactivity in the mice was mainly eliminated through the kidneys. The uptake of 125 I-VIP in the carcinoma, muscles, lungs, liver and intestines was inhibited with nonlabelled VIP in a dose-dependent manner. The ratio of 10 μg and 40 μg of VIP with T/NT was 26.87% and 35.82% respectively. Conclusion The combination of 125 I-VIP with H22 hepatocarcinoma is mediated with VIP receptors of the H22 cells in vitro and in the body of mice which provides the theoretic foundation for the further study of targetting hepatocarcinoma with radionuclide-labelled VIP." @default.
- W2364163722 created "2016-06-24" @default.
- W2364163722 creator A5062199142 @default.
- W2364163722 date "2005-01-01" @default.
- W2364163722 modified "2023-09-26" @default.
- W2364163722 title "Study of binding of ~(125)I-VIP with VIP receptor in H22 hepatocarcinoma cells in mice" @default.
- W2364163722 hasPublicationYear "2005" @default.
- W2364163722 type Work @default.
- W2364163722 sameAs 2364163722 @default.
- W2364163722 citedByCount "0" @default.
- W2364163722 crossrefType "journal-article" @default.
- W2364163722 hasAuthorship W2364163722A5062199142 @default.
- W2364163722 hasConcept C107824862 @default.
- W2364163722 hasConcept C118303440 @default.
- W2364163722 hasConcept C126322002 @default.
- W2364163722 hasConcept C134018914 @default.
- W2364163722 hasConcept C153911025 @default.
- W2364163722 hasConcept C163994747 @default.
- W2364163722 hasConcept C170493617 @default.
- W2364163722 hasConcept C178790620 @default.
- W2364163722 hasConcept C181199279 @default.
- W2364163722 hasConcept C185592680 @default.
- W2364163722 hasConcept C202751555 @default.
- W2364163722 hasConcept C2777807558 @default.
- W2364163722 hasConcept C2781060987 @default.
- W2364163722 hasConcept C55493867 @default.
- W2364163722 hasConcept C71924100 @default.
- W2364163722 hasConcept C8330315 @default.
- W2364163722 hasConcept C86803240 @default.
- W2364163722 hasConceptScore W2364163722C107824862 @default.
- W2364163722 hasConceptScore W2364163722C118303440 @default.
- W2364163722 hasConceptScore W2364163722C126322002 @default.
- W2364163722 hasConceptScore W2364163722C134018914 @default.
- W2364163722 hasConceptScore W2364163722C153911025 @default.
- W2364163722 hasConceptScore W2364163722C163994747 @default.
- W2364163722 hasConceptScore W2364163722C170493617 @default.
- W2364163722 hasConceptScore W2364163722C178790620 @default.
- W2364163722 hasConceptScore W2364163722C181199279 @default.
- W2364163722 hasConceptScore W2364163722C185592680 @default.
- W2364163722 hasConceptScore W2364163722C202751555 @default.
- W2364163722 hasConceptScore W2364163722C2777807558 @default.
- W2364163722 hasConceptScore W2364163722C2781060987 @default.
- W2364163722 hasConceptScore W2364163722C55493867 @default.
- W2364163722 hasConceptScore W2364163722C71924100 @default.
- W2364163722 hasConceptScore W2364163722C8330315 @default.
- W2364163722 hasConceptScore W2364163722C86803240 @default.
- W2364163722 hasLocation W23641637221 @default.
- W2364163722 hasOpenAccess W2364163722 @default.
- W2364163722 hasPrimaryLocation W23641637221 @default.
- W2364163722 hasRelatedWork W1527731832 @default.
- W2364163722 hasRelatedWork W1579169096 @default.
- W2364163722 hasRelatedWork W170011754 @default.
- W2364163722 hasRelatedWork W1902800299 @default.
- W2364163722 hasRelatedWork W1979070849 @default.
- W2364163722 hasRelatedWork W1981587320 @default.
- W2364163722 hasRelatedWork W2022156307 @default.
- W2364163722 hasRelatedWork W2030986366 @default.
- W2364163722 hasRelatedWork W2032180125 @default.
- W2364163722 hasRelatedWork W2040664813 @default.
- W2364163722 hasRelatedWork W2040886527 @default.
- W2364163722 hasRelatedWork W2055559597 @default.
- W2364163722 hasRelatedWork W2056302832 @default.
- W2364163722 hasRelatedWork W2123497769 @default.
- W2364163722 hasRelatedWork W2203275373 @default.
- W2364163722 hasRelatedWork W2343888053 @default.
- W2364163722 hasRelatedWork W2400206204 @default.
- W2364163722 hasRelatedWork W2409931022 @default.
- W2364163722 hasRelatedWork W2412645781 @default.
- W2364163722 hasRelatedWork W2521623166 @default.
- W2364163722 isParatext "false" @default.
- W2364163722 isRetracted "false" @default.
- W2364163722 magId "2364163722" @default.
- W2364163722 workType "article" @default.