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- W2364164890 abstract "A novel piezoelectric immunosensor for Schistosoma-Japonicum(Sj) has been developed, on the basis of a new biomolecular immobilization matrix using self-assembled cystamine film and SiO2 nanoparticles. The newly prepared SiO2 nanoparticles exhibited excellent physical and chemical properties, such as high surface-to-area and biocompatibility. After the surface functionalization, they were utilized to load Sj antigens(SjAg) at a larger scale. The formed sensitized nanoparticles of SjAg@SiO2 were immobilized onto the sensor that was modified with self-assembled cystamine film, resulting in a new piezoelectric immunosensor for detecting Sj antibodies(SjAb). The results showed that the three-dimensional(3D) spacial structures of SiO2 nanoparticle matrix could facilitate enhanced antigen-antibody immunoreaction, achieving high sensitivity for the detection of SjAb analytes. Under the optimized experimental conditions, the developed piezoelectric immunosensor was proved be capable of quantitatively determining SjAb in the concentration (dilution ratio) range of 0.6~22.7 μg/mL with a determination limit of 0.4 μg/mL(S/N=3). Moreover, the new immunoassay was used for the analysis of clinical samples. It could show favorable performances compared to the classical enzyme-linked immunosorbnent assay (ELISA), indicating a promising alternative technique for schistosoma-japonicum in clinical diagnosis, field applications and epidemic situation monitoring." @default.
- W2364164890 created "2016-06-24" @default.
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- W2364164890 date "2009-01-01" @default.
- W2364164890 modified "2023-09-24" @default.
- W2364164890 title "A Piezoelectric Schistosoma-Japonicum Immunosensor Based on Self-Assembled Cystamine Film and SiO_2 Nanoparticles" @default.
- W2364164890 hasPublicationYear "2009" @default.
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