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- W2364167978 abstract "This inveatigation is to develop a analysis method for the simultaneous determination of caffeic acid and rosmarinci acid in Herba Lycopi by HPLC.Fourteen samples from different habitats and collecting time were analyzed.The samples were extracted with 30%(volumetric percentage) ethanol by ultrasonic.The separation was perfomed by a Hypersil ODS2(5 μm,4.6 mm i.d.×250 mm) at a flow rate of 1.0 mL/min with the detection wavelength at UV325 nm.The mobile phase consisted of acetonitrile with formic acid(0.75%)(A) and water with formic acid(0.75%)(B).The gradient profile was that solution A was held 10% for 5min and then linearly from 10% to 55% in 65 min.The injection volume was 20 μL and the column temperature was maintained at 30 ℃.The highest mass fraction of caffeic acid and rosmarinci acid were detected in Herba Lycopi from Hebei among ten habitats of Hebei,Shandong,Jiangsu,Jiangxi,Guangxi,Hubei,Hunan,Anhui,Gansu,Beijing,and the sample collected in August from Beijing showed the highest content among five samples collected from June to October.The method is sensitive and accurate with good repeatability and suitable for quality control of Herba Lycopi." @default.
- W2364167978 created "2016-06-24" @default.
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- W2364167978 date "2009-01-01" @default.
- W2364167978 modified "2023-09-25" @default.
- W2364167978 title "Simultaneous Determination of Caffeic Aicd and Rosmarinci Acid in Herba Lycopi by High-performance Liquid Chromatography" @default.
- W2364167978 hasPublicationYear "2009" @default.
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