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- W2364179660 abstract "In the present research,sequence of myostatin(MSTN)gene and its 5'-flanking region was amplified from genome DNA isolated from genetically improved farmed tilapia(GIFT)with PCR and genome walking technique.The whole sequence with the length of 2413 bp included 5'-flanking region(462 bp),three exons and two introns.The sequence length of exon Ⅰ,exon Ⅱ,and partial exon Ⅲ was 379 bp,371 bp,and 145 bp,respectively.And the sequence length of intronⅠand intron Ⅱ was 305bp and 751bp respectively.The open reading frame encoded 298 amino acids.Sequence analysis of the 5'-flanking region revealed that it contained putative muscle growth response elements(E-box)and other transcription regulation elements such as TATA box,OCT1,AP1 and AP4.Three SNPs were obtained through sequencing MSTN gene from 10 random GIFTs.However only one SNP in intronⅡshowed polymorphism through testing from total samples.Meanwhile,growth traits of body weight,length,height and thick of GIFT(45♂,51♀)were measured,and the association between growth traits and SNPs polymorphism in MSTN gene was analyzed.The results showed that GIFT's body shape parameters(including body width/body length and body height/body length)had a significant correlation with the SNP locating in MSTN intron Ⅱ 728 nt(P0.05).In short,the SNPs(T/G)of MSTN could be used as a candidate molecular marker in GIFT breeding." @default.
- W2364179660 created "2016-06-24" @default.
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- W2364179660 date "2010-01-01" @default.
- W2364179660 modified "2023-09-25" @default.
- W2364179660 title "Genetic structure of MSTN and association between its polymorphisms and growth traits in genetically improved farmed tilapia (GIFT)" @default.
- W2364179660 hasPublicationYear "2010" @default.
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