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- W2364183765 abstract "Objective:To assign phenolic acids and determine the content of chlorogenic acid contained in Fructus Xanthii collected from different product areas,and establish a practical quality control method for the herbal drug.Method: Major phenolic acids of this herb were identified based on their UV absorption,retention time and mass fragmentation characteristics.The content of chlorogenic acid was determined using ultra-performance liquid chromatography-ultraviolet detector(UPLC-UV).The mobile phase consists of 0.1% phosphoric acid in water as solvent A and methanol as solvent B.The detection wavelength is set at 327 nm and the column temperature at 35 ℃.The mobile phase flow rate is 0.4 mL·min-1.Result: A total of 9 major chromatographic peaks were assigned as phenolic acids,and peak area vs.content of chlorogenic acid showed a good linearity in the range of 3.5-350 mg·L-1 with a correlation coefficient of 0.999 9 and the average recovery was 101.7%.Conclusion:The established method is suitable for determining the content of chlorogenic acid contained in Fructus Xanthii and can be used to effectively control the quality of the herb;and assigning major characteristic peaks can significantly enhance specificity and accuracy in accessing the quality of herb." @default.
- W2364183765 created "2016-06-24" @default.
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- W2364183765 date "2011-01-01" @default.
- W2364183765 modified "2023-09-25" @default.
- W2364183765 title "Characterization of Phenolic Acids and Quantitative Analysis of Chlorogenic Acid Contained in Fructus Xanthii" @default.
- W2364183765 hasPublicationYear "2011" @default.
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