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- W2364185619 abstract "In previous study, a novel gene, LRRC4, a member of leucine rich repeat(LRR) superfamily was cloned. Expression analysis indicated that LRR may play an important role in the central nervous system. To investigate the function and the structure function relationship of LRRC4, full length coding region was amplified and subcloned into pGEM T Easy vector. Further, the recombinant plasmid, pEGFP C1/LRRC4, was constructed and transfected transiently into U251 cell. Under the fluorescence microscope, the green fluorescence produced by LRRC4 fusion protein was observed on the cytoplasmic membrane. Consistent to prediction by bioinformatics, this result indicated that product of LRRC4 is a membrane protein. In addition, the recombinant of LRRC4,pGEX 4T 2/LRRC4 and truncated LRRC4 recombinant, pGEX 4T 2/mLRRC4, were constructed and transformed into E.coli BL21. Induced by 0 5 mmol/L IPTG, The band corresponding to fusion protein were observed in SDS PAGE as expected. Together with bioinformatic analysis of LRRC4 protein, these results establish the basis for functional study of LRRC4." @default.
- W2364185619 created "2016-06-24" @default.
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- W2364185619 date "2002-01-01" @default.
- W2364185619 modified "2023-09-27" @default.
- W2364185619 title "Preliminary Study of LRRC4 Protein: Bioinformatic Analysis, Fusion Expression in Eukaryote and Prokaryote" @default.
- W2364185619 hasPublicationYear "2002" @default.
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