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- W2364186589 abstract "Total RNA was extracted from the second stage larva of H.lineatum.Hypodermin A gene of H.lineatum was amplified by RT-PCR.The target gene was cloned and sequenced.The prokaryotic expression vector pET30-HA was constructed and then transformed to E.coli BL21(DE3).The recombinant E.coli was induced and expressed by IPTG.The expression level of the specific protein was more than 35% of total protein.It was mainly present in inclusion body observed by SDS-PAGE.Western blot analysis indicated that the obtained protein was recombinant hypodermin A,and had good reactiogenicity against positive serum." @default.
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- W2364186589 date "2008-01-01" @default.
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- W2364186589 title "Expression of hypodermin A gene of H. lineatum in Escherichia coli" @default.
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