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- W2364188212 abstract "Objective To evaluate the transfection efficiency of novel non-viral nano gene vector polyethyleneimine-β-cyclodextrin(PEI-β-CyD) on chondrocyte cell line C5.18 and bone marrow-derived mesenchymal stem cell line C3H10T1/2.Methods Chondrocyte cell line C5.18 and bone marrow-derived mesenchymal stem cell line C3H10T1/2 were cultured in vitro,and the cytotoxicity of PEI-β-CyD and PEI25KDa(polyethyleneimine of 25 000 in molecular weight) on C5.18 cells and C3H10T1/2 cells was observed and compared by MTT method.C5.18 cells and C3H10T1/2 cells were transfected with PEI-β-CyD and PEI25KDa respectively under different ratios of effective nitrogen content in vectors to effective phosphorus content in exogenous gene(N/P),and the transfection efficiency was analysed by inverted fluorescent microscopy and flow cytometry.Results The cytotoxicity of PEI-β-CyD was significantly lower than that of PEI25KDa to both C5.18 cells and C3H10T1/2 cells.For C5.18 cells,there was no significant difference in transfection efficiency between PEI-β-CyD and PEI25KDa(P0.05),and for C3H10T1/2 cells,transfection efficiency of PEI-β-CyD was significantly higher than that of PEI25KDa(P0.05).Conclusion PEI-β-CyD is an ideal non-viral nano gene vector with low cytotoxicity and favorable transfection efficiency for chondrocyte cell line C5.18 and bone marrow-derived mesenchymal stem cell line C3H10T1/2,which is promising in the research and application of cartilage tissue engineering and cell therapy." @default.
- W2364188212 created "2016-06-24" @default.
- W2364188212 creator A5018308873 @default.
- W2364188212 date "2011-01-01" @default.
- W2364188212 modified "2023-09-23" @default.
- W2364188212 title "Evaluation of transfection efficiency of novel non-viral nano gene vector PEI-β-CyD on chondrocytes and bone marrow-derived mesenchymal stem cells" @default.
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