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- W2364191725 abstract "Object To discuss the development of a “bar coded” HPLC chromatographic fingerprint system for the assay of water soluble components in Radix Salvia Miltiorrhiza (RSM) The technique has been used for the species authentication and quality assessment of RSM and other species of Salvia L. originated from different locations.Methods Authentic RSM species originated from different geographic locations were compared with different species of Salvia L. obtained commercially using an analytical protocol involving water extraction, followed by HPLC of the extract, and finally the normalization of all peak intensities in the HPLC chromatogram. The last step resulted in “bar coded” chromatograms which could be used as fingerprints for the qualitative and quantitative comparison of different species of Salvia L Results The HPLC bar code fingerprint was found to be highly stable and reproducible. A total of 18 common peaks were identified in the HPLC chromatogram with their average RSD of relative retention time and peak area less than 1.18% and 25%, respectively. Separated by the protocatechuic aldehyde peak (retention time 33.71 min and RSD of 1.5%), the HPLC chromatogram could be separated into two groups: the early eluting group of peaks in the “non specific” region, and the late eluting group of peaks in the “fingerprinting” region. The former group of peaks could be used for the semi quantification of bioactive components, whereas the later group of peaks could be used for both identification and semi quantification purposes. HPLC chromatograms of other species of Salvia L. showed fingerprints distinctively different from those of authentic species of Salvia L.; and the difference lay mainly in the fingerprinting region of the HPLC chromatograms Conclusion The developed bar coded HPLC chromatographic fingerprints can be used effectively for the authentication of species of Salvia L., and the quality assessment of RSM or its derived products based on the semi quantitative analysis of bioactive components in the samples" @default.
- W2364191725 created "2016-06-24" @default.
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- W2364191725 date "2003-01-01" @default.
- W2364191725 modified "2023-09-23" @default.
- W2364191725 title "Development of HPLC fingerprint bar code technique for authentication and quality assessment of Radix Salvia Miltiorrhiza" @default.
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