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- W2364191737 abstract "Objective:To study on the thin-layer chromatographic identification method and the assay method for garlic in Chinese Pharmacopoeia(2010)with reference to USP,EP and BP.Method: The enzyme activity was inhibited by micro-wave,and garlic was identified by thin-layer chromatography.The content of alliin in garlic was performed on a cation exchange chromatography column,with the phosphate buffered solution as the mobile phase,at the flow rate of 0.5 mL·min-1 and the detected wavelength was 214 nm.Results: The thin-layer chromatograms of garlic from different regions showed spots corresponding in Rf value to that obtained from the chromatograms of standard solution alliin and arginine.The method experiment of the established HPLC to detect the content of alliin showed that the calibration curve of alliin was linear in the range of 31.2-374.4 μg·mL-1(r=0.9999)and the average recoveries(n=9)at low,medium and high concentration were 102.8%(RSD=4.0%),101.8%(RSD=3.0%),111.0%(RSD=3.8%),respectively.Conclusion: The TLC identification and assay methods for garlic were established in this study.The methods can be used in the quantitative and qualitative analysis of garlic,which was suggested to be referenced in the further study of the quality specification of garlic." @default.
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- W2364191737 date "2012-01-01" @default.
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- W2364191737 title "Study on the discussion about quality specification of garlic" @default.
- W2364191737 hasPublicationYear "2012" @default.
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