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- W2364191864 abstract "Specific primers were designed according to the sequences of human and bovine BMP4 genes published on GenBank to clone the encoding sequence,intron2,and intron 3 of BMP4 gene in Huanghuai goat.In addition,the cDNA encoding the mature protein of goat BMP4 was amplified from the full coding DNA sequence,then it was cloned into the expression vector pET32a,and transformed into the host cell E.coli BL21(DE3)plysS.The inductive condition for expression was optimized.The results showed that the length of encoding sequence,intron2 and 3 of goat BMP4 were 1 230 bp,1 053 bp and 962 bp,respectively.Corresponding sequence comparisons of goat with those of pig,human,mouse and cattle demonstrated that the homologies of the nucleotides were 96.10%,94.96%,91.79% and 99.19% for the coding DNA sequences;83.80%,76.03%,66.94% and 96.71% for the intron 2 and 87.15%,80.24%,67.20%,98.13% for the intron 3,respectively.The molecular weight of goat BMP4 fusion protein was 31 KD,and the optimized condition for the inductive expression was at 22℃,0.5 mmol/L IPTG,for 8h induction.Thses results suggested that the full-length encoding sequence and the sequences of intron2 and intron3 of goat BMP4 gene have been successively cloned.The determination of optimized condition for prokaryotic expression supplies a basis for the further studies on the biological role of goat BMP4." @default.
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- W2364191864 date "2010-01-01" @default.
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- W2364191864 title "Cloning and optimized expression of bone morphogenetic protein4 (BMP4) gene in Huanghuai goat" @default.
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