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- W2364195468 abstract "Objective: To analyze NF-κB gene expression in peripheral blood mononuclear cell and explore the clinical implications in diagnosis and prognosis of hepatocellular carcinoma(HCC).Methods: Total RNAs were extracted from peripheral blood mononuclear cells(PBMC).The gene fragment of NF-κB /p65 mRNA was amplified by a real-time PCR with absolute standard,and confirmed by its sequencing,and the copies of circulating NF-κB gene were measured quantitatively and their clinical values were analyzed.Results: The copies of NF-κB/p65 mRNA in the PBMCs from HCC patients were significantly higher than those in any other groups with benign liver diseases.The amplified fragment of NF-κB gene,identified by sequencing,was in accordance with its original sequence.The copies of NF-κB/p65 mRNA were obviously decreased in HCC patients after resection,and obviously increased again with recidivist HCC patients.Conclusion: NF-κB is overexpressed in HCC,and the analysis of NF-κB mRNA in PBMCs has better sensitivity and specificity.NF-κB is a useful marker for diagnosing HCC or monitoring metastasis and relapse and is a potential molecular target for HCC gene therapy." @default.
- W2364195468 created "2016-06-24" @default.
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- W2364195468 date "2010-01-01" @default.
- W2364195468 modified "2023-10-06" @default.
- W2364195468 title "Expression level of circulating NF-κB gene and its clinical implications in patients with hepatocelluar carcinoma" @default.
- W2364195468 hasPublicationYear "2010" @default.
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