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- W2364201077 abstract "Objective To investigate the expression level of endogenetic nitric oxide(NO) in the reproduction process of human anterior cruciate ligament cells. Methods Anterior cruciate ligament cells were isolated and subcultured from the human anterior cruciate ligament. LPS was used to induce the anterior cruciate ligament cell to express the inducible nitric oxide synthase(iNOS ), and N-monomethyl -L-Arginine (L-NMMA) was used as the interdiction of nitric oxide. They were alone or together added in the culture medium of anterior cruciate ligament cells in different groups. The level of NO was indirectly measured in the medium of HACL. Results LPS promoted significantly anterior cruciate ligament cells to produce endogenetic nitric oxide, compared with the control group(P 0.05). L-NMMA inhibited significantly anterior cruciate ligament cells to produce endogenetic nitric oxide,compared with the control group(P 0.05). The level of NO in the group used both LPS and L-NMMA was significantly different from that in the control group(P 0.05). Conclusion Endogenetic nitric oxide play significantly a role in the reproduction process of human anterior cruciate ligament cells under the inducement from LPS, and L-NMMA may inhibit the production of endogenetic nitric oxide which decrease the reproduction of human anterior cruciate ligament cells." @default.
- W2364201077 created "2016-06-24" @default.
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- W2364201077 date "2005-01-01" @default.
- W2364201077 modified "2023-09-28" @default.
- W2364201077 title "Expression level of endogenetic nitric oxide on reproduction of human anterior cruciate ligament cells" @default.
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