Matches in SemOpenAlex for { <https://semopenalex.org/work/W2364201635> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2364201635 abstract "The extraction and purification of R-phycoerythrin(PE) in the thallus of Porphyra yezoensis was studied. The different cell fragmentation methods(freezing/unfreezing method, chemistry method, dissolving-bulging method, etc, and their admixture cell fragmentation methods) were applied and compared. The results showed that the combination of freezing/unfreezing adding tissue-trituration method was the optimal method for extracting phycoerythrin. The purification of crude extracts was performed by means of ammonium sulphate precipitation in different concentrations(25%～60%) and the phycoerythrin purification(D565nm/D280nm) was checked. The results showed that most phycoerythrin could precipitate between 25% and 45% concentration of (NH4)2SO4. So the 25% and 45% concentration of (NH4)2SO4 was applied to remove small protein and precipitated the phycoerythrin respectively. The purity of R-PE reached up to 1.09 and then increased to 2.09 by crystallization. The remained ammonium sulfate was removed by chromatography on a Sephadex G-25 column pre-equilibrated with the same buffer. The results showed that the purity of R-PE reached 2.97. Then the R-PE was further purified through hydroxylapatite(HA) column and the purity of R-PE reached up to 4.98. There was a maximum absorption peak at 565 nm, and a fluorescence emission peak at 578 nm. SDS-PAGE for R-PE showed that there were two subunits, α subunit and β subunit, with 17.0 kD and 19.0 kD of molecular weights respectively." @default.
- W2364201635 created "2016-06-24" @default.
- W2364201635 creator A5073669273 @default.
- W2364201635 date "2005-01-01" @default.
- W2364201635 modified "2023-09-25" @default.
- W2364201635 title "Extraction and Purification of R-Phycoerythrin in Porphyra yezoensis" @default.
- W2364201635 hasPublicationYear "2005" @default.
- W2364201635 type Work @default.
- W2364201635 sameAs 2364201635 @default.
- W2364201635 citedByCount "2" @default.
- W2364201635 crossrefType "journal-article" @default.
- W2364201635 hasAuthorship W2364201635A5073669273 @default.
- W2364201635 hasConcept C137243819 @default.
- W2364201635 hasConcept C153911025 @default.
- W2364201635 hasConcept C163994747 @default.
- W2364201635 hasConcept C178790620 @default.
- W2364201635 hasConcept C181199279 @default.
- W2364201635 hasConcept C185592680 @default.
- W2364201635 hasConcept C193363816 @default.
- W2364201635 hasConcept C2776428949 @default.
- W2364201635 hasConcept C2777914628 @default.
- W2364201635 hasConcept C2778177487 @default.
- W2364201635 hasConcept C2779870022 @default.
- W2364201635 hasConcept C2780925461 @default.
- W2364201635 hasConcept C43617362 @default.
- W2364201635 hasConcept C4725764 @default.
- W2364201635 hasConcept C553184892 @default.
- W2364201635 hasConcept C55493867 @default.
- W2364201635 hasConcept C559758991 @default.
- W2364201635 hasConcept C59822182 @default.
- W2364201635 hasConcept C86803240 @default.
- W2364201635 hasConceptScore W2364201635C137243819 @default.
- W2364201635 hasConceptScore W2364201635C153911025 @default.
- W2364201635 hasConceptScore W2364201635C163994747 @default.
- W2364201635 hasConceptScore W2364201635C178790620 @default.
- W2364201635 hasConceptScore W2364201635C181199279 @default.
- W2364201635 hasConceptScore W2364201635C185592680 @default.
- W2364201635 hasConceptScore W2364201635C193363816 @default.
- W2364201635 hasConceptScore W2364201635C2776428949 @default.
- W2364201635 hasConceptScore W2364201635C2777914628 @default.
- W2364201635 hasConceptScore W2364201635C2778177487 @default.
- W2364201635 hasConceptScore W2364201635C2779870022 @default.
- W2364201635 hasConceptScore W2364201635C2780925461 @default.
- W2364201635 hasConceptScore W2364201635C43617362 @default.
- W2364201635 hasConceptScore W2364201635C4725764 @default.
- W2364201635 hasConceptScore W2364201635C553184892 @default.
- W2364201635 hasConceptScore W2364201635C55493867 @default.
- W2364201635 hasConceptScore W2364201635C559758991 @default.
- W2364201635 hasConceptScore W2364201635C59822182 @default.
- W2364201635 hasConceptScore W2364201635C86803240 @default.
- W2364201635 hasLocation W23642016351 @default.
- W2364201635 hasOpenAccess W2364201635 @default.
- W2364201635 hasPrimaryLocation W23642016351 @default.
- W2364201635 hasRelatedWork W2019606143 @default.
- W2364201635 hasRelatedWork W2042499840 @default.
- W2364201635 hasRelatedWork W2064467561 @default.
- W2364201635 hasRelatedWork W2105330731 @default.
- W2364201635 hasRelatedWork W2283235478 @default.
- W2364201635 hasRelatedWork W2350897313 @default.
- W2364201635 hasRelatedWork W2353821025 @default.
- W2364201635 hasRelatedWork W2354454463 @default.
- W2364201635 hasRelatedWork W2379082991 @default.
- W2364201635 hasRelatedWork W2389440705 @default.
- W2364201635 hasRelatedWork W2390181987 @default.
- W2364201635 hasRelatedWork W2800201229 @default.
- W2364201635 hasRelatedWork W2908074598 @default.
- W2364201635 hasRelatedWork W2912175280 @default.
- W2364201635 hasRelatedWork W3047083960 @default.
- W2364201635 hasRelatedWork W3109092393 @default.
- W2364201635 hasRelatedWork W3147298810 @default.
- W2364201635 hasRelatedWork W2181727449 @default.
- W2364201635 hasRelatedWork W2818618397 @default.
- W2364201635 hasRelatedWork W2880511807 @default.
- W2364201635 isParatext "false" @default.
- W2364201635 isRetracted "false" @default.
- W2364201635 magId "2364201635" @default.
- W2364201635 workType "article" @default.