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- W2364203466 abstract "To develop a specific assay for detection of Pseudomonas plecoglossicida, the SYBR green I real-time PCR assay were established with a pair of primers targeting rpo D gene of P.plecoglossicida. Then, a standard curve was constructed with series dilution of the recombinant plasmids containing the rpo D gene. The correlation coefficient of standard curve constructed using the threshold cycle(CT) versus log10 copy numbers of standard plasmid samples showed a good linearity(R2=0.999), and the minimum level of detection was 1.9 ×103copies/μL. The specificity of the assay was confirmed by the lack of cross-amplification with DNA templates from P.putica, Vibrio alginolyticus, V.parahaemolyticus, Streptococcus iniae, Aeromonas hydrophilia, V.vulnificus, Shcwanella alga, P.fluorescens and Nocardia seriolae. The coefficients of variance(CV) were 1.29% and 1.70% for the intra- and inter-assay, respectively, which indicated a good reliability and reproducibility. This detection assay provided a rapid means to quantitatively accessing the presence of P.plecoglossicida, which can be a useful tool for the quantification and monitoring of this bacterial pathogen in farmed fish, as well as the diagnosis of the P.plecoglossicida-related fish disease." @default.
- W2364203466 created "2016-06-24" @default.
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- W2364203466 date "2015-01-01" @default.
- W2364203466 modified "2023-09-24" @default.
- W2364203466 title "Development of a SYBR GreenIreal-time PCR assay for specific detection of Pseudomonas plecoglossicida" @default.
- W2364203466 hasPublicationYear "2015" @default.
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