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- W2364207308 abstract "Objective By means of primary culture of ameloblasts and observation with light and electron microscope, we are to make a base for the progressive study on the development and influencing factors of ameloblasts in vitro. Methods 7 - day - old rats were used to get their lower incisors. 0.25% collagenase and 0.25% trypsin were used to disperse the cells. DMEM were used for the cells' culture, and a light and an electorn microscope were used for the cells' observation. Results After nine to ten days' culture, the cells grew well and contacted each other and gathered to clusters. The bodies of the ameloblasts were a little columnar like. And there were many organellae which developed very well in the cytoplasm. Conclusion The ameloblasts in vitro could keep their features in vivo. The method we had used could be used for the study of ameloblasts in vitro." @default.
- W2364207308 created "2016-06-24" @default.
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- W2364207308 date "2002-01-01" @default.
- W2364207308 modified "2023-09-25" @default.
- W2364207308 title "A Primary Culture of Rat Ameloblasts and Observation with Light and Electron Microscope" @default.
- W2364207308 hasPublicationYear "2002" @default.
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