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- W2364225405 abstract "Objective: To construct a yciD deletion mutant of Shigella flexneri 2a strain 2457T,in order to explore the function of yciD of S.flexneri 2a 2457T.Methods: In according to complete genome sequence of S.flexneri 2a 2457T,the yciD gene was deleted by using Red recombination system,and confirmed by PCR and SDS-PAGE.The growth of mutant and wild strain were measured separately,and some of biochemical events of mutant and wild strain were comparatively investigated.Results: The yciD deletion mutant strain 2457TΔyciD of S.flexneri 2a 2457T was constructed.The protein band,which molecular weight was about 22 000 corresponding to sequence deduced from yciD gene,was absent in sample of outer membrane protein.The mutant strain grew faster than wild strain,and their capability to utilize glucose and mannitol was more than wild strain.Conclusion: The yciD deletion mutant strain of S.flexneri 2a 2457T was obtained." @default.
- W2364225405 created "2016-06-24" @default.
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- W2364225405 date "2006-01-01" @default.
- W2364225405 modified "2023-09-23" @default.
- W2364225405 title "Construction of yciD Deletion Mutant of Shigella flexneri 2a Strain 2457T" @default.
- W2364225405 hasPublicationYear "2006" @default.
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