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- W2364228712 abstract "PCR technology has been one important part of modern biological laboratories after it was established about 20 years ago.How to obtain a good result,however,relies on the operator's experience to an extent,because it is possessed complicated kinetic process and influenced by various factors.Starting from the analysis of the reaction kinetics,we depicted the process of product accumulation for the PCR amplification.Furthermore,we constructed an iterative model for simulating the PCR.We evaluated effect of various factors on PCR amplification,i.e.,the amount of template,cycling numbers,amount of polymerase,the half-life of polymerase,and denaturation time with each amplification cycle.Finally,we performed various PCR experiments with different treatments respectively,including amounts of template,cycling number,amounts of polymerase,and the template mixture.The results indicated that excellent results could be obtained with moderate amount of template,with polymerase in higher quality,and with moderate cycle times.The key factor that determined results of various PCR is primers,but various adjustments for PCR operation possess of accessorial effects only." @default.
- W2364228712 created "2016-06-24" @default.
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- W2364228712 date "2007-01-01" @default.
- W2364228712 modified "2023-09-23" @default.
- W2364228712 title "Kinetic Simulation and Amplification Effects Resulted From Various Treatments for Polymerase Chain Reaction(PCR)" @default.
- W2364228712 hasPublicationYear "2007" @default.
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