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- W2364238058 abstract "Blastoderm cells modified genetically from stage X embryos of chicken could still enter recipient embryos at the same stage of development and were the able to produce chimeras. Donor cells suspended from stage X blastoderms and recombinant plasmid P GS1 containing the infectious bronchitis virus S1 gene were embedded in liposome and co cultured. The co culture was then treated in three ways: 1. Microinjected into the receptors ( n =140)at the same stage of development directly. 2. The co culture was anti screened with G418 before being microinjected. 3. In order to produce chimeras donor cells were cultured in a monolayer in vitro for 48 h then co cultured with recombinant plasmid P GS1 before being microinjected into the receptors( n =190). PCR and RAPD analyses of DNA from the blood of young chickens and embryonic tissue indicated that the hatchability of the first group(5 3%) was significantly higher than that of the second(1 4%) and the third (2 1%) (One Way ANOVA, P 0 05). The positive rate of exogenous DNA amplification in embryonic organs from the second group was higher than that from the first or third group. These results indicate that donor cells cultured in vitro for 48 h and co cultured with plasmid DNA might migrate into recipient embryonic tissue to produce chimeras. It is, therefore, potentially possible to produce transgenic chickens using lipofection mediated exogenous gene transfer to blastoderm cells isolated from stage X embryos. Subsequent injection of these cells into receptors at the same stage of development could result in the production of germline chimeras." @default.
- W2364238058 created "2016-06-24" @default.
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- W2364238058 date "2002-01-01" @default.
- W2364238058 modified "2023-09-23" @default.
- W2364238058 title "CULTURE OF CHICKEN STAGE X BLASTODERM CELLS" @default.
- W2364238058 hasPublicationYear "2002" @default.
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