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- W2364243053 abstract "To construct the Recombinant goatpoxvirus expressing keywrod's ontside membrance protein(OMP25) gene of B.melitensis,the OMP25 gene was inserted into the downstream of promotor I1L of goatpoxvirus(GPV)transfer vector PGM-TK-I1L-GPT1.Then the recombinant transfer vector was transfected into sheep fibroblast cells infected with GPV vaccine strain G14-STV44-55 by lipofectamine.The transfected sheep fibroblast cells were conducted by mycophenolic acid(MPA)to screen and purify recombinant goatpoxvirus(RGPV).Recombinant goatpoxvirus was successfully obtained and identified by PCR." @default.
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- W2364243053 date "2008-01-01" @default.
- W2364243053 modified "2023-09-23" @default.
- W2364243053 title "Construction of the Recombinant Goatpoxvirus Expressing OMP25 Gene of B.melitensis" @default.
- W2364243053 hasPublicationYear "2008" @default.
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