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- W2364247367 abstract "Objective To study the regulation of M2 receptor mRNA stability by active component of Zhimu, ZDY101. Methods After the addition of ZDY101 or vehicle to the cultured CHOm2 cells, the synthesis of M2 receptor mRNA in the cells were stopped by the addition of actinomycin D, and then the time course of the M2 receptor mRNA was quantified by real-time quantitative PCR. The slope and curve of the decline of M2 receptor mRNA was traced, and the half-life was obtained. Results The results showed that before the addition of actinomycin D, the M2 receptor mRNA level in the ZDY101 treated group was higher than the vehicle treated group. After the addition of actinomycin D, the decline of the M2 receptor mRNA was significantly slower and the half-life was significantly longer in the ZDY101 treated group than the vehicle treated group. Conclusion The increase of M2 receptor density was at least partly attributable to the increase of M2 mRNA stability caused by ZDY101." @default.
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- W2364247367 date "2005-01-01" @default.
- W2364247367 modified "2023-09-23" @default.
- W2364247367 title "Study on the regulation of M2 mRNA stability by active component of Zhimu, ZDY101" @default.
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