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- W2364252034 abstract "Objective To construct and identify recombinant adenoviruses containing fusion genes of ubiquitin(Ub) and Hantaan virus Gn or Gc and S0.7. Methods The fragment of Ub was amplified by PCR and inserted into the p Shuttle-p CAG-Gn S0.7 and p Shuttle-p CAG-Gc S0.7 to create new transfer vectors p Shuttle-p CAG-Ub-Gn S0.7 and p Shuttle-p CAG-Ub-Gc S0.7, respectively. The p CAG-Ub-Gn S0.7 and p CAG-Ub-Gc S0.7 fragments were then cloned into Adeno-XTMViral vector using PI-Sce I and I-Ceu I digestion. The recombinant adenovirus vectors, r Ad-p CAG-Ub-Gn S0.7 and r Ad-p CAG-Ub-Gc S0.7 linearized by Pac Ⅰ were transfected into HEK 293 cells to package the recombinant adenoviruses. Finally, the expression of target proteins in HEK 293 cells were detected by indirect immunofluorescence assay(IFA) after being infected by recombinant adenoviruses. Results The recombinant transfer vectors and recombinant adenovirus vectors were confirmed by enzyme digestion, target gene PCR and sequencing. The expressions of target proteins of recombinant adenoviruses were successfully detected by IFA. Conclusions The recombinant adenoviruses containing fusion genes of Ub, Hantaan virus Gn or Gc and S0.7 are successfully constructed, which can be used in further study on the immunological characteristics of the Hantaan virus recombinant adenoviruses." @default.
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- W2364252034 date "2015-01-01" @default.
- W2364252034 modified "2023-09-23" @default.
- W2364252034 title "Construction and identification of recombinant adenoviruses containing fusion genes of ubiquitin and Hantaan virus Gn or Gc and S0.7" @default.
- W2364252034 hasPublicationYear "2015" @default.
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