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- W2364255448 abstract "Aim To investigate the mechanism for the inhibitory effect of hydrogen sulfide on the expression of tissue factor(TF) induced by oxidative low-density lipoprotein(ox-LDL) in endothelial cells. Methods Human umbilical vein endothelial cells(HUVECs)were treated with 50 mg·L- 1ox-LDL in the absence or presence of different concentrations of NaHS(25,50,100 and 200 μmol ·L- 1) for 24 h. The mRNA expression and protein content of TF in HUVECs were determined by reverse transcription PCR and ELISA,respectively. The content of intracellular reactive oxygen species(ROS) was determined by DCFH,an oxidative sensitive fluorescent indicator. The activation of nuclear factor-kappaB(NF-κB) was estimated by its expression in nuclear extracts analyzed by Western blot. Results Ox-LDL induced TF mRNA expression and increased TF protein content in HUVECs. The increase in intracellular ROS production and the activation of NF-κB were observed in HUVECs treated with ox-LDL. However,NaHS could markedly inhibit the increases in TF mRNA and protein levels induced by ox-LDL. Also the elevation of intracellular ROS production and the activation of NF-κB elicited by ox-LDL were significantly suppressed by pretreatment with NaHS. In addition,pretreatment with BAY 11-7082(10 μmol·L- 1),the inhibitor of NF-κB or N-acetylL-cysteine(1 mmol·L- 1),an antioxidant,could also decrease the TF mRNA and protein level as well as ROS production and NF-κB activation induced by oxLDL in HUVECs,similar to the effects of 200 μmol·L- 1NaHS. Conclusion The mechanism for the inhibitory effect of H2S on the ox-LDL- induced TF expression in endothelial cells may be related to inhibiting intracellular ROS production and subsequently NF-κB activation." @default.
- W2364255448 created "2016-06-24" @default.
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- W2364255448 date "2014-01-01" @default.
- W2364255448 modified "2023-09-23" @default.
- W2364255448 title "Exogenous hydrogen sulfide inhibits expression of tissue factor induced by ox-LDL through reducing generation of ROS and inhibiting NF-κB activation in endothelial cells" @default.
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