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- W2364255512 abstract "In order to determine the cause of death of a Sichuan golden monkey(Rhinopithecus roxellanao), Necropsies were carried out according to a standard protocol. Tissue samples were fixed in 10% neutral buffered formalin solution and embedded in paraffin. The 4 μm sections were deparaffinized, stained and processed routinely for pathohistology analysis. Most samples were examined for detecting bacteriums by inoculating into the broth medium and viruses by Vero, PK-15, BHK-21 cells culture, morphology and reverse transcription-polymerase chain reaction (RT-PCR). Then the KM mice regression test were carried out by the intraperitoneal injection with different isolated strains. The results showed that Cardiac pericardium, epicardium, and endocardium appeared bleeding point and ecchymosis; cardiac muscle appeared white necrotic focus; lungs were swollen and congestive. Cardiac muscle fiberes were abound with inflammatory cell infiltrate; parts of them were broke, degenerated. The heart appeared typical myocarditis changes in which the blood capillary of alveolar wall highly stretched and congested. The viruses (Picornaviridae-like morphous) and bacteria (Bacillus coli) were isolated from the samples of golden monkey. The KM mice regression test showed that the bacteria had no pathogenicity on mice. However, the viruses isolated could make mice infected, and it also showed the similar pathologic symptoms with the Sichuan golden monkey. The RT-PCR performed on an extract of cardiac muscle tissue revealed a DNA sequence specific for Coxsackievirus B. Our results indicated that the myocarditis caused by the Coxsackievirus B resulted in Sichuan golden monkey’s death." @default.
- W2364255512 created "2016-06-24" @default.
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- W2364255512 date "2008-01-01" @default.
- W2364255512 modified "2023-09-23" @default.
- W2364255512 title "Diagnosis and etiopathogenesis analysis on Sichuan golden monkey with myocarditis" @default.
- W2364255512 hasPublicationYear "2008" @default.
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