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- W2364264549 abstract "OBJECTIVE:To study the effects of flow components C6 and C7 in n-butyl alcohol extract from the leaves of Cestrum Nocturnum(CN)on the proliferation and apoptosis of human gastric cancer cell SGC7901. METHODS:C6 and C7 were obtained by using different ratio of chloroform and methanol(1 ∶ 9,1 ∶ 7)to the gradient elution of CN leaves. After cultured with 0(blank control),5,10,20,40,80 μg/ml C6 and C7 for 72 h,inhibitory effect of C6 and C7 on the proliferation of SGC7901 was determined by MTT assay. Inhibitory rate and IC50 were calculated. After SGC7901 were cultured with 10 μg/ml C6 and C7 for 72 h,colony formation assay was utilized to detect the effects of C6 and C7 on the cell colony formation,and the rate of colony formation was calculated. In addition,Wright/Giemsa and Hoechst33258/PI staining assay were used to observe the change of cytomorphology. RESULTS:MTT showed that C6 and C7 had inhibitory effect on the proliferation of SGC7901 to different extent;inhibitory rates were 22.1%-80.0% and 19.6%-79.7%,and IC50 were 16.4,18.05 μ g/ml,respectively. Compared with blank control group,colony formation rate of C6 and C7 group decreased,with statistical significance(P0.05). The number of apoptotic cells was more in treatment group than in other groups. CONCLUSIONS:Flow components C6 and C7 in n-butyl alcohol extract from the leaves of CN can inhibit the proliferation of SGC7901 cells and induce the apoptosis of them." @default.
- W2364264549 created "2016-06-24" @default.
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- W2364264549 date "2015-01-01" @default.
- W2364264549 modified "2023-09-23" @default.
- W2364264549 title "Effects of Flow Components C6 and C7 in N-butyl Alcohol Extract from the Leaves of Cestrum nocturnum on the Proliferation and Apoptosis of Human Gastric Cancer Cell SGC7901" @default.
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