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- W2364272987 abstract "In this study,a Phi class GSTnamed PtoGSTF4was cloned fromPopulus tomentosa.PtoGSTF4 encodes a protein of 213amino acid residues.RT-PCR revealed that PtoGSTF4was a constitutively expressed gene that is expressed in stem,leaf and phloem of stem of P.tomentosaunder normal growth condition,and H2 O2 and atrazine stressed conditions.The recombinant PtoGSTF4was expressed in E.coli and purified by Ni 2+sepharose affinity chromatography.PtoGSTF4showed enzymatic activities towards substrates CDNB,NBD-Cl,NBC and Cum-OOH.Kinetic analysis found that the affinity of PtoGSTF4to GSH was higher than that to CDNB.PtoGSTF4had optimal pH ranging from 7.5~10.5and optimal temperature ranging from 30℃~60 ℃.Our results indicated that PtoGSTF4may play important roles in stress tolerance in P.tomentosa." @default.
- W2364272987 created "2016-06-24" @default.
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- W2364272987 date "2013-01-01" @default.
- W2364272987 modified "2023-09-23" @default.
- W2364272987 title "Cloning and Characterization of PtoGSTF4 from Populus tomentosa" @default.
- W2364272987 hasPublicationYear "2013" @default.
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